Elucidating mechanism cellular uptake minerals used in isotopic dating

As is well known, it is difficult for nucleic acids to diffuse directly through plasma membrane due to their size and/or their physicochemical properties, for instance, hydrophilicity.

Various strategies for the transfer of nucleic acids, especially genes, have thus been developed [8-10].

Table 2 Detailed information and cell types (in nine cell lines) with relatively higher luciferase gene transfection efficiency without fetal calf serum of six commercially available transfection reagents.

This review also briefly introduces applications of these chemical methods for gene delivery. Nanoscopic structure of DNA condensed for gene delivery. Evaluation of proinflammatory cytokine production induced by linear and branched polyethylenimine/plasmid DNA complexes in mice. A two-stage poly(ethylenimine)-mediated cytotoxicity: implications for gene transfer/therapy. Nanjing Maternal and Child Health Medical Institute, Nanjing Maternal and Child Health Hospital Affiliated of Nanjing Medical University, Nanjing 210029, China3. Economical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province, Hunan Key Laboratory of Green Packaging and Biomedical Nanotechnology, Hunan University of Technology, Zhuzhou 412007, China* These two authors contribute to this paper equally. Keywords: Non-viral, Gene delivery, Vectors, Chemical Methods. A variety of genetic mutations, which alter cellular proliferation, angiogenesis, metastasis, and tumor immunogenicity result in human cancer, which has become one of the biggest threats to human life [1]. A novel non-viral vector for DNA delivery based on low molecular weight, branched polyethylenimine: effect of molecular weight on transfection efficiency and cytotoxicity.

Leave a Reply